Journal: Scientific Reports

Article Title: Role of methionine adenosyltransferase 2A in bovine preimplantation development and its associated genomic regions

doi: 10.1038/s41598-017-04003-1

Figure Lengend Snippet: Immunofluorescence analysis of MAT2A protein in bovine oocytes and preimplantation embryos. Confocal images that transverse at least one nucleus or pronucleus are shown. ( A ) Immature oocyte, ( B ) mature oocyte, ( C ) 1-cell, ( D ) 2-cell, ( E ) 8-cell, ( F ) morula, ( G ) blastocyst, ( H ) hatched blastocyst, and ( I ) negative control in which the primary antibody was omitted from the immunofluorescence of a hatched blastocyst sample. Scale bars represent 50 µm. ( A’ – I’ ) Nuclear counterstaining with propidium iodide. ( J ) and ( K ) Immunoblotting of 1-cell embryo (n = 120) and blastocyst (n = 120) lysates using the monoclonal MAT2A antibody used in the immunofluorescence and ChIP-seq analyses. Hep G2 Cell Lysate (50 µg protein) was used as a positive control.

Article Snippet: The MAT2A inhibitor FIDAS was purchased from Merck Millipore and dissolved in dimethyl sulfoxide (DMSO) at 20 mM and stored at −20 °C until further use.

Techniques: Immunofluorescence, Negative Control, Western Blot, ChIP-sequencing, Positive Control

Journal: Scientific Reports

Article Title: Role of methionine adenosyltransferase 2A in bovine preimplantation development and its associated genomic regions

doi: 10.1038/s41598-017-04003-1

Figure Lengend Snippet: Data generated by ChIP-seq analysis of bovine blastocyst using MAT2A antibody.

Article Snippet: The MAT2A inhibitor FIDAS was purchased from Merck Millipore and dissolved in dimethyl sulfoxide (DMSO) at 20 mM and stored at −20 °C until further use.

Techniques: Generated, Control

Journal: Scientific Reports

Article Title: Role of methionine adenosyltransferase 2A in bovine preimplantation development and its associated genomic regions

doi: 10.1038/s41598-017-04003-1

Figure Lengend Snippet: Characterization of MAT2A-ChIP specific peaks. ( A ) Chromosome distribution of 76 MAT2A-ChIP specific peaks. The figure was generated using the web-based CEAS ( http://liulab.dfci.harvard.edu/CEAS/ ) tool. ( B ) Distribution of the peak-associated genes in terms of where the central point of the peak is located among different genomic regions. The bovine genome was divided into the following four categories: upstream (<100 kb) of a transcription start site (TSS), downstream (<100 kb) of a transcription end site (TES), the intron, and intergenic (>100 kb from a TSS or TES) regions. The numbers indicate those of genes for the upstream, intron, and downstream regions and those of peaks for the intergenic regions, respectively. ( C ) ChIP-qPCR validation of several MAT2A-associated peaks. Relative enrichments to IgG control are shown as the mean value of two to five ChIP-qPCR analyses for each peak. The peaks are shown with ID number (Table ) and their associated genes in parentheses. The GAPDH promoter was used as a negative control for enrichment.

Article Snippet: The MAT2A inhibitor FIDAS was purchased from Merck Millipore and dissolved in dimethyl sulfoxide (DMSO) at 20 mM and stored at −20 °C until further use.

Techniques: Generated, ChIP-qPCR, Biomarker Discovery, Control, Negative Control

Journal: Scientific Reports

Article Title: Role of methionine adenosyltransferase 2A in bovine preimplantation development and its associated genomic regions

doi: 10.1038/s41598-017-04003-1

Figure Lengend Snippet: Gene ontology (GO) annotation in terms of biological process enriched from 35 genes associated with the MAT2A-ChIP specific peaks. All significant (P < 0.05) secondary level terms obtained as output from a web-based GO analysis toolkit, AgriGO ( http://bioinfo.cau.edu.cn/agriGO/ ), are shown. Blue columns show the percentage of genes among the peak-associated genes, and green columns show the percentage of genes within the whole genome.

Article Snippet: The MAT2A inhibitor FIDAS was purchased from Merck Millipore and dissolved in dimethyl sulfoxide (DMSO) at 20 mM and stored at −20 °C until further use.

Techniques: